6XT1
The structure of the M60 catalytic domain from Clostridium perfringens ZmpC in complex the sialyl T antigen
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-002 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-09-07 |
Detector | DECTRIS PILATUS 200K |
Wavelength(s) | 1.54187 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 109.615, 100.930, 119.024 |
Unit cell angles | 90.00, 95.91, 90.00 |
Refinement procedure
Resolution | 39.070 - 2.490 |
R-factor | 0.2368 |
Rwork | 0.234 |
R-free | 0.27970 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6xsz |
RMSD bond length | 0.002 |
RMSD bond angle | 0.702 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.540 |
High resolution limit [Å] | 2.490 | 2.500 |
Rmerge | 0.109 | 0.589 |
Rpim | 0.051 | 0.317 |
Number of reflections | 89691 | 4505 |
<I/σ(I)> | 15.3 | 2.3 |
Completeness [%] | 99.9 | 100 |
Redundancy | 4.2 | 4 |
CC(1/2) | 0.988 | 0.899 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 291 | 7.6% Tascimate, 19% PEG 3350 |