6XQI

Structure of HIV-1 Vpr in complex with the human nucleotide excision repair protein hHR23A

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 31 2 1
Unit cell lengths	130.165, 130.165, 81.305
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.783 - 2.340
R-factor	0.2022
Rwork	0.202
R-free	0.21970
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.15.1_3469)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	24.783	24.783	2.390
High resolution limit [Å]	2.340	9.390	2.340
Rmerge	0.114	0.041	9.900
Rmeas	0.125	0.045	14.774
Number of reflections	41494	478	2424
<I/σ(I)>	6.46	24.14	0.1
Completeness [%]	89.4	82.1	71.5
Redundancy	4.508	5.584	1.838
CC(1/2)	0.998	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	289	0.1M Tris, pH 7.5, 10% PEG 4000