6XQ1
Receptor for Advanced Glycation End Products VC1 domain in complex with 3-(3-((4-(4-carboxyphenoxy)benzyl)oxy)phenyl)-1H-indole-2-carboxylic acid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-04-18 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.97857 |
Spacegroup name | P 62 |
Unit cell lengths | 102.180, 102.180, 102.407 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 33.450 - 1.510 |
R-factor | 0.1732 |
Rwork | 0.172 |
R-free | 0.19650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4lp4 |
RMSD bond length | 0.017 |
RMSD bond angle | 1.460 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.450 | 1.550 |
High resolution limit [Å] | 1.510 | 1.510 |
Rmeas | 0.061 | 0.783 |
Rpim | 0.020 | 0.284 |
Number of reflections | 95300 | 9586 |
<I/σ(I)> | 16.4 | 2.19 |
Completeness [%] | 99.8 | 99.4 |
Redundancy | 4.7 | 3.8 |
CC(1/2) | 0.965 | 0.808 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 2.5 M NaOAc (pH 7.5) |