6XPZ

Human antibody S1V2-83 in complex with the influenza hemagglutinin head domain of A/Moscow/10/1999(H3N2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	80
Detector technology	PIXEL
Collection date	2018-10-23
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97910
Spacegroup name	P 21 2 21
Unit cell lengths	85.790, 103.710, 220.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.100 - 3.450
R-factor	0.2533
Rwork	0.251
R-free	0.30680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB entries 5W08 6dc3 5sx4 & 6E4X
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.100	3.573
High resolution limit [Å]	3.450	3.450
Rmerge	0.212	1.149
Rmeas	0.225	1.216
Rpim	0.073	0.395
Number of reflections	26508	2582
<I/σ(I)>	10.01	2.06
Completeness [%]	99.5	99.08
Redundancy	9	9.2
CC(1/2)	0.996	0.837

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	5% w/v PEG4000