6XOG
Structure of SUMO1-ML786519 adduct bound to SAE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-05-29 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.97949 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 57.900, 72.422, 126.870 |
Unit cell angles | 90.00, 93.86, 90.00 |
Refinement procedure
Resolution | 47.702 - 1.980 |
Rwork | 0.206 |
R-free | 0.24000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1y8r |
RMSD bond length | 0.009 |
RMSD bond angle | 1.617 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.702 | 2.050 |
High resolution limit [Å] | 1.980 | 1.980 |
Number of reflections | 71980 | 7068 |
<I/σ(I)> | 25.7 | 2.37 |
Completeness [%] | 98.5 | 97.15 |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | 50 mM BisTris, pH 6.5, 50 mM Ammonium Sulfate, 30% pentaerythritol ethoxylate (Hampton Index 57) |