6XOC

Crystal structure of glVRC01 Fab in complex with anti-idiotypic iv4 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1
Spacegroup name	I 2 2 2
Unit cell lengths	100.016, 114.417, 220.969
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.010 - 2.450
R-factor	0.2008
Rwork	0.199
R-free	0.23980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6mft
RMSD bond length	0.010
RMSD bond angle	0.992
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	110.500	2.540
High resolution limit [Å]	2.450	2.450
Number of reflections	46804	46727
<I/σ(I)>	17
Completeness [%]	99.4
Redundancy	8.9
CC(1/2)	0.990	0.785

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M Tris pH 7.5, 8%w/v PEG 20K, 8%w/v PEG MME 550 and 0.2 M KSCN