6XNK
Crystal structure of dimeric K72A human cytochrome c alkaline conformer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-18 |
| Detector | DECTRIS PILATUS3 X 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 |
| Unit cell lengths | 35.660, 49.595, 61.556 |
| Unit cell angles | 77.97, 79.02, 90.19 |
Refinement procedure
| Resolution | 42.110 - 2.080 |
| R-factor | 0.1815 |
| Rwork | 0.176 |
| R-free | 0.23350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ty3 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.405 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.120 | 2.150 |
| High resolution limit [Å] | 2.080 | 2.080 |
| Rmerge | 0.140 | 0.600 |
| Number of reflections | 22075 | 1757 |
| <I/σ(I)> | 3.05 | |
| Completeness [%] | 90.5 | 72.4 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.9 | 293 | 30% MPD, 0.1 M CHES pH 9.9 |
