6XM8
Crystal structure of lignostilbene bound to Co-LSD4 from Sphingobium sp. strain SYK-6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08B1-1 |
Synchrotron site | CLSI |
Beamline | 08B1-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-07-07 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.97934 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 85.700, 112.540, 115.520 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.305 - 1.850 |
R-factor | 0.1479 |
Rwork | 0.147 |
R-free | 0.17320 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6ojr |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX (1.14-3260) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.305 | 40.305 | 1.960 |
High resolution limit [Å] | 1.850 | 5.000 | 1.850 |
Rmerge | 0.154 | 0.073 | 0.730 |
Rmeas | 0.180 | 0.085 | 0.854 |
Total number of observations | 351935 | ||
Number of reflections | 92163 | 4679 | 14633 |
<I/σ(I)> | 6.66 | 14.05 | 2.13 |
Completeness [%] | 100.0 | 99.8 | 100 |
Redundancy | 3.819 | 3.831 | 3.793 |
CC(1/2) | 0.988 | 0.990 | 0.708 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.3 M magnesium acetate, 29% PEG3350 |