6XL1

crystal structure of cA4-activated Card1(D294N)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-16
Detector	PSI PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	P 41 21 2
Unit cell lengths	111.946, 111.946, 172.180
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.580 - 1.950
R-factor	0.1791
Rwork	0.178
R-free	0.20160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6wwx
RMSD bond length	0.008
RMSD bond angle	0.843
Data reduction software	autoXDS
Data scaling software	autoXDS
Phasing software	PHASER
Refinement software	PHENIX (1.18_3855)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.020
High resolution limit [Å]	1.950	1.950
Rmerge	0.081	0.661
Rmeas	0.083	0.684
Rpim	0.021	0.174
Number of reflections	80132	7872
<I/σ(I)>	23.41	4.08
Completeness [%]	99.9	99.94
Redundancy	15	15.2
CC(1/2)	0.998	0.936

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	0.1 M HEPES 7.0, 30% (v/v) Jeffamine M-600