6XGV
Crystal Structure of KRAS-G13D (GMPPNP-bound) in complex with RAS-binding domain (RBD) and cysteine-rich domain (CRD) of RAF1/CRAF
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-02-22 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9791 |
Spacegroup name | P 6 2 2 |
Unit cell lengths | 132.750, 132.750, 89.480 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.360 - 2.110 |
R-factor | 0.1859 |
Rwork | 0.184 |
R-free | 0.20430 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6xhb |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.360 | 48.360 | 2.230 |
High resolution limit [Å] | 2.100 | 6.250 | 2.100 |
Rmerge | 0.082 | 0.033 | 1.172 |
Rmeas | 0.086 | 0.034 | 1.217 |
Total number of observations | 354688 | ||
Number of reflections | 27312 | 1169 | 4174 |
<I/σ(I)> | 18.69 | 55.17 | 2.16 |
Completeness [%] | 99.1 | 97.3 | 95.9 |
Redundancy | 12.987 | 11.366 | 13.631 |
CC(1/2) | 0.999 | 0.999 | 0.824 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 100 mM sodium cacodylate pH 6.5, 700 mM sodium acetate, 0.35 mM D-myo-phosphatidylinositol 3,4,5-triphosphate |