6XFR
Metallo-beta-lactamase from Pontibacter korlensis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-03-29 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.95372 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 97.941, 97.941, 103.237 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.245 - 2.608 |
R-factor | 0.1792 |
Rwork | 0.176 |
R-free | 0.23550 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4c09 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.970 | 2.600 |
High resolution limit [Å] | 2.600 | 2.600 |
Number of reflections | 15978 | 15976 |
<I/σ(I)> | 0.08 | |
Completeness [%] | 99.8 | |
Redundancy | 1 | |
CC(1/2) | 0.997 | 0.997 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293.15 | 0.1 M Tris, 0.002 M ZnCl2, 20% w/v PEG 6000 |