6XF1

Nesprin-2G(aa1425-1649)-FHOD1(aa1-339) complex, H. sapiens

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	80
Detector technology	CCD
Collection date	2017-12-10
Detector	ADSC QUANTUM 315
Wavelength(s)	0.987
Spacegroup name	C 1 2 1
Unit cell lengths	237.825, 52.443, 177.458
Unit cell angles	90.00, 123.27, 90.00

Refinement procedure

Resolution	67.270 - 2.800
R-factor	0.2193
Rwork	0.218
R-free	0.26160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3dad
RMSD bond length	0.013
RMSD bond angle	1.951
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	67.300	2.900
High resolution limit [Å]	2.800	2.800
Rpim	0.071
Number of reflections	50981	4500
<I/σ(I)>	21.6
Completeness [%]	98.9
Redundancy	5.9
CC(1/2)	0.990	0.540

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		292	0.1 M Tris/HCl pH 8.5, 0.2 M potassium sodium tartrate, 21% PEG 3350