6XER

Tubulin-RB3_SLD in complex with colchicine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2019-09-13
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	65.717, 127.025, 254.208
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.340 - 2.500
R-factor	0.1696
Rwork	0.168
R-free	0.22260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xiw
RMSD bond length	0.007
RMSD bond angle	0.933
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.550
High resolution limit [Å]	2.500	2.500
Rmerge	0.119	0.911
Rpim	0.058	0.505
Number of reflections	75107	4642
<I/σ(I)>	16.6	1.4
Completeness [%]	99.7	99.7
Redundancy	4.6	4
CC(1/2)	0.990	0.646

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.6	293	PEG 4000, ammonium sulfate, tri-sodium citrate