6XCV
Crystal structure of apo SznF from Streptomyces achromogenes var. streptozoticus NRRL 2697
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-10-20 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.03319 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 57.907, 108.132, 150.229 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.220 - 1.770 |
R-factor | 0.2084 |
Rwork | 0.206 |
R-free | 0.25440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6m9r |
Data reduction software | HKL-3000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.800 |
High resolution limit [Å] | 1.770 | 1.770 |
Rmerge | 0.063 | |
Number of reflections | 92848 | 4473 |
<I/σ(I)> | 26.5 | |
Completeness [%] | 90.6 | |
Redundancy | 5.7 | |
CC(1/2) | 0.937 | 0.698 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 16% PEG4000, 0.1 M magnesium chloride, 0.1 M Tris, pH 8.5 |