6XCV

Crystal structure of apo SznF from Streptomyces achromogenes var. streptozoticus NRRL 2697

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.03319
Spacegroup name	P 21 21 21
Unit cell lengths	57.907, 108.132, 150.229
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.220 - 1.770
R-factor	0.2084
Rwork	0.206
R-free	0.25440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6m9r
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.800
High resolution limit [Å]	1.770	1.770
Rmerge	0.063
Number of reflections	92848	4473
<I/σ(I)>	26.5
Completeness [%]	90.6
Redundancy	5.7
CC(1/2)	0.937	0.698

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	16% PEG4000, 0.1 M magnesium chloride, 0.1 M Tris, pH 8.5