6XCO

Immune receptor complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 1 21 1
Unit cell lengths	69.288, 104.310, 69.316
Unit cell angles	90.00, 114.91, 90.00

Refinement procedure

Resolution	40.140 - 2.900
R-factor	0.2086
Rwork	0.207
R-free	0.24710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4z7u
RMSD bond length	0.004
RMSD bond angle	0.563
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.15_3459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.140	3.004
High resolution limit [Å]	2.900	2.900
Rmerge	0.085	0.402
Rmeas	0.121	0.568
Rpim	0.085	0.402
Number of reflections	19889	1983
<I/σ(I)>	5.7	1.94
Completeness [%]	99.9
Redundancy	2
CC(1/2)	0.985	0.724

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293.15	0.1 M calcium acetate, 0.1 M sodium cacodylate pH 5.5, 13% polyethylene glycol (PEG) 20,000