6XCO
Immune receptor complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-26 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.288, 104.310, 69.316 |
| Unit cell angles | 90.00, 114.91, 90.00 |
Refinement procedure
| Resolution | 40.140 - 2.900 |
| R-factor | 0.2086 |
| Rwork | 0.207 |
| R-free | 0.24710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4z7u |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.563 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15_3459) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.140 | 3.004 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.085 | 0.402 |
| Rmeas | 0.121 | 0.568 |
| Rpim | 0.085 | 0.402 |
| Number of reflections | 19889 | 1983 |
| <I/σ(I)> | 5.7 | 1.94 |
| Completeness [%] | 99.9 | |
| Redundancy | 2 | |
| CC(1/2) | 0.985 | 0.724 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293.15 | 0.1 M calcium acetate, 0.1 M sodium cacodylate pH 5.5, 13% polyethylene glycol (PEG) 20,000 |
