6XBB

Crystal structure of Streptomyces sviceus SsDesB in complex with NADP+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2019-07-12
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	84.125, 153.433, 141.314
Unit cell angles	90.00, 92.44, 90.00

Refinement procedure

Resolution	37.016 - 2.366
R-factor	0.1919
Rwork	0.189
R-free	0.23950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5o8p
Data reduction software	HKL-2000
Data scaling software	HKL-3000
Phasing software	HKL-3000
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.410
High resolution limit [Å]	2.366	2.366
Rmerge	0.059	0.580
Rmeas	0.103	0.809
Rpim	0.089	0.418
Number of reflections	139487	6996
<I/σ(I)>	14.2	2
Completeness [%]	96.3
Redundancy	3.7
CC(1/2)	0.996	0.729

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	0.1M HEPES pH 7.5, 0.2M NaCl, 20% PEG3350, 10 mM sarcosine