6XB8
Adeno-Associated Virus Origin Binding Domain in complex with ssDNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2011-03-10 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 170.180, 173.220, 173.450 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.860 - 3.300 |
| R-factor | 0.2019 |
| Rwork | 0.201 |
| R-free | 0.21760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5byg |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.820 |
| Data reduction software | MOSFLM |
| Data scaling software | pointless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.18rc2_3794) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.450 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.125 | 0.594 |
| Number of reflections | 38830 | 4683 |
| <I/σ(I)> | 15.5 | 5.42 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 10 | 9.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 10mM Na cacodylate, pH 6.5, 30% PEG400 and 0.2M LiCl |
