6XB8
Adeno-Associated Virus Origin Binding Domain in complex with ssDNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X6A |
Synchrotron site | NSLS |
Beamline | X6A |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2011-03-10 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9795 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 170.180, 173.220, 173.450 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.860 - 3.300 |
R-factor | 0.2019 |
Rwork | 0.201 |
R-free | 0.21760 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5byg |
RMSD bond length | 0.005 |
RMSD bond angle | 0.820 |
Data reduction software | MOSFLM |
Data scaling software | pointless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.18rc2_3794) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.450 |
High resolution limit [Å] | 3.300 | 3.300 |
Rmerge | 0.125 | 0.594 |
Number of reflections | 38830 | 4683 |
<I/σ(I)> | 15.5 | 5.42 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 10 | 9.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 10mM Na cacodylate, pH 6.5, 30% PEG400 and 0.2M LiCl |