6XB5

Structure of Trichoplusia ni poxin in post-reactive state with Gp[2'-5']Ap[3']

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2019-05-24
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.99995
Spacegroup name	I 41
Unit cell lengths	124.422, 124.422, 132.178
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.570 - 2.900
R-factor	0.1869
Rwork	0.184
R-free	0.21460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Phased with Companion Structure 6XB6
RMSD bond length	0.006
RMSD bond angle	0.934
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.570	3.070
High resolution limit [Å]	2.890	2.890
Rpim	0.068	0.854
Number of reflections	22422	3527
<I/σ(I)>	9.5	1.1
Completeness [%]	99.6	97.9
Redundancy	15.2	14.7
CC(1/2)	0.996	0.372

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291	100 mM HEPES-KOH, 32% Jeffamine ED-2001