6XAZ

Structure of the PR domain from human PRDM5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL14-1
Synchrotron site	SSRL
Beamline	BL14-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.19
Spacegroup name	P 21 21 21
Unit cell lengths	47.527, 56.083, 105.307
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.830 - 1.700
Rwork	0.211
R-free	0.25930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4c1q
RMSD bond length	0.012
RMSD bond angle	1.289
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.14)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.830	1.760
High resolution limit [Å]	1.700	1.700
Number of reflections	31092	2929
<I/σ(I)>	32.6	2.4
Completeness [%]	98.1
Redundancy	9.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.1 M sodium cacodylate pH 6.5, 2.0 M ammonium sulfate, 0.2 M NaCl