6X88
PDE6 chicken GAF domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-10-23 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1 |
Spacegroup name | P 65 |
Unit cell lengths | 148.499, 148.499, 93.678 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 38.980 - 3.200 |
R-factor | 0.214852968782 |
Rwork | 0.210 |
R-free | 0.30731 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 2.148 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.981 | 3.260 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.143 | 0.890 |
Number of reflections | 19554 | 906 |
<I/σ(I)> | 6.2 | |
Completeness [%] | 86.0 | |
Redundancy | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 2 uL 8 mg/mL purified chicken cone GAFab protein + 2 uL well buffer (0.1 M HEPES sodium, pH 7.5, 21-27% PEG3350, 0.2 M ammonium sulfate, 20% glycerol) |