6X5U
Human Alpha-1,6-fucosyltransferase (FUT8) bound to GDP and NM5M2-Asn
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-03-01 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 65 |
| Unit cell lengths | 154.020, 154.020, 466.920 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 87.840 - 3.200 |
| R-factor | 0.1943 |
| Rwork | 0.190 |
| R-free | 0.23590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6x5h |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.550 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 87.842 | 3.280 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmeas | 0.259 | 1.400 |
| Number of reflections | 102079 | 7647 |
| <I/σ(I)> | 5.7 | 1.2 |
| Completeness [%] | 99.3 | 99.9 |
| Redundancy | 3.6 | 3.7 |
| CC(1/2) | 0.985 | 0.509 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 10% PEG 3350, 0.2 M Ammonium chloride |
