6X4T

Crystal structure of ICOS-L in complex with Prezalumab and VNAR domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.033158
Spacegroup name	P 1 21 1
Unit cell lengths	68.508, 151.984, 86.675
Unit cell angles	90.00, 104.10, 90.00

Refinement procedure

Resolution	38.880 - 3.150
R-factor	0.2035
Rwork	0.201
R-free	0.25620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1i8l 4i0k 1t6v internal database
RMSD bond length	0.004
RMSD bond angle	0.807
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.250
High resolution limit [Å]	3.150	3.150
Rmerge	0.170	0.670
Rpim	0.068	0.270
Number of reflections	29746	1378
<I/σ(I)>	9.4	1.7
Completeness [%]	99.9	100
Redundancy	7	7
CC(1/2)	0.990	0.760

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.2 M di-ammonium tartrate and 20% (w/v) PEG 3350