6X4I
Crystal Structure of NSP15 Endoribonuclease from SARS CoV-2 in the Complex with 3'-uridinemonophosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-05-20 |
Detector | DECTRIS PILATUS3 X 6M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 63 |
Unit cell lengths | 150.940, 150.940, 111.858 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.190 - 1.850 |
R-factor | 0.1669 |
Rwork | 0.166 |
R-free | 0.18890 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6vww |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.880 |
High resolution limit [Å] | 1.850 | 5.020 | 1.850 |
Rmerge | 0.146 | 0.067 | 1.587 |
Rmeas | 0.152 | 0.070 | 1.720 |
Rpim | 0.041 | 0.019 | 0.648 |
Total number of observations | 1578786 | ||
Number of reflections | 122922 | 6288 | 6120 |
<I/σ(I)> | 6.3 | ||
Completeness [%] | 100.0 | 100 | 99.7 |
Redundancy | 12.8 | 13.7 | 6.4 |
CC(1/2) | 0.998 | 0.561 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 289 | 0.2M Sodium Chloride, 0.1M Sodium Potassium phosphate, 10% PEG8000 |