6X2D
Crystal Structure of DNase I Domain of Ribonuclease E from Vibrio cholerae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2020-03-17 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97872 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 40.614, 58.812, 47.046 |
Unit cell angles | 90.00, 108.16, 90.00 |
Refinement procedure
Resolution | 29.410 - 1.850 |
R-factor | 0.2085 |
Rwork | 0.207 |
R-free | 0.24290 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2vrt |
RMSD bond length | 0.005 |
RMSD bond angle | 1.292 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.880 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.088 | 0.759 |
Rmeas | 0.097 | 0.833 |
Rpim | 0.041 | 0.339 |
Number of reflections | 17863 | 897 |
<I/σ(I)> | 21.9 | 4.7 |
Completeness [%] | 99.4 | 100 |
Redundancy | 5.7 | 6 |
CC(1/2) | 0.985 | 0.927 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 292 | Protein: 7.9 mg/ml, 0.01M Tris pH 8.3; Screen: PACT (F3), 0.2M Sodium iodide, 0.1M Bis-Tris propane pH 6.5, 20% (w/v) PEG 3350 |