6X1Q
1.8 Angstrom resolution structure of b-galactosidase with a 200 kV cryoARM electron microscope
EM specimen
| Vitrification | Yes |
| Instrument | LEICA EM GP |
EM Data Collection
| Microscope | JEOL CRYO ARM 200 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2.7 |
| Energy Filter | In-column Omega Filter |
| Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 1.8 |
| Number of Particle | 998945 |
| Software | RELION,SerialEM,CTFFIND,UCSF Chimera |
| Symmetry | D2 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
