6WZQ
Structure of SARS-CoV-2 Nucleocapsid dimerization domain, P21 form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-05-12 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 71.146, 43.546, 74.233 |
Unit cell angles | 90.00, 94.87, 90.00 |
Refinement procedure
Resolution | 73.970 - 1.450 |
R-factor | 0.168 |
Rwork | 0.167 |
R-free | 0.18020 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6wxo |
RMSD bond length | 0.009 |
RMSD bond angle | 0.922 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 74.000 | 1.470 |
High resolution limit [Å] | 1.450 | 1.450 |
Rmerge | 0.058 | 0.802 |
Rmeas | 0.063 | 0.877 |
Number of reflections | 80163 | 3906 |
<I/σ(I)> | 16 | 2 |
Completeness [%] | 98.8 | |
Redundancy | 6.6 | |
CC(1/2) | 0.998 | 0.796 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 100 mM Tris-HCl pH 8.5, 50 mM Ammonium Sulfate, and 38% polyethylene glycol (PEG) 3350 |