6WYF

Crystal structure of Human H-chain Ferritin variant 157C Delta C-star Modified with a RAFT Agent Soaked in an Acrylate Solution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	F 4 3 2
Unit cell lengths	179.940, 179.940, 179.940
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.730 - 1.250
R-factor	0.1142
Rwork	0.113
R-free	0.13070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6b8f
RMSD bond length	0.009
RMSD bond angle	1.065
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.250	1.270
High resolution limit [Å]	1.250	1.250
Number of reflections	64146	3357
<I/σ(I)>	29.5	2.7
Completeness [%]	93.0
Redundancy	59.1
CC(1/2)	1.000	0.791

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	5 uL reservoir + 5 uL 25 uM 157C delta C-star Human H-chain Ferritin in 15 mM HEPES, pH 7.0 against reservoir of 500 uL 25 mM HEPES, pH 8.0, 10 mM calcium chloride, 140 mM sodium chloride