6WWC
Vaccine-elicited mouse FP-targeting neutralizing antibody vFP16.02 with S48K mutation in light chain in complex with HIV fusion peptide (residue 512-519)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-10-02 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 68.628, 70.526, 93.292 |
Unit cell angles | 90.00, 94.79, 90.00 |
Refinement procedure
Resolution | 35.263 - 2.563 |
R-factor | 0.2117 |
Rwork | 0.209 |
R-free | 0.26650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6cdo |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.640 |
High resolution limit [Å] | 2.500 | 7.050 | 2.600 |
Rmerge | 0.124 | 0.045 | 0.438 |
Rmeas | 0.150 | 0.054 | 0.527 |
Rpim | 0.083 | 0.029 | 0.289 |
Number of reflections | 23890 | 1227 | 1152 |
<I/σ(I)> | 5.2 | ||
Completeness [%] | 97.5 | 95.2 | 94.2 |
Redundancy | 3.1 | 3.1 | 2.9 |
CC(1/2) | 0.996 | 0.857 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Tris-HCl, pH 8.5, 30% v/v PEG400 |