6WVV
Plasmodium vivax M17 leucyl aminopeptidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-28 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 117.035, 201.549, 166.224 |
| Unit cell angles | 90.00, 106.01, 90.00 |
Refinement procedure
| Resolution | 44.320 - 2.330 |
| R-factor | 0.2073 |
| Rwork | 0.205 |
| R-free | 0.24350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kqz |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.497 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.950 | 2.330 |
| High resolution limit [Å] | 2.290 | 2.290 |
| Rmerge | 0.154 | |
| Rmeas | 0.174 | |
| Rpim | 0.081 | |
| Number of reflections | 327356 | 15478 |
| <I/σ(I)> | 5.9 | |
| Completeness [%] | 99.7 | 95.3 |
| Redundancy | 4.5 | 4 |
| CC(1/2) | 0.992 | 0.408 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 17% PEG 3350, 0.1 M HEPES pH 6.5, 0.15 M (NH4)2SO4 |
