6WVV

Plasmodium vivax M17 leucyl aminopeptidase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-28
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 1 21 1
Unit cell lengths	117.035, 201.549, 166.224
Unit cell angles	90.00, 106.01, 90.00

Refinement procedure

Resolution	44.320 - 2.330
R-factor	0.2073
Rwork	0.205
R-free	0.24350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kqz
RMSD bond length	0.003
RMSD bond angle	0.497
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.950	2.330
High resolution limit [Å]	2.290	2.290
Rmerge	0.154
Rmeas	0.174
Rpim	0.081
Number of reflections	327356	15478
<I/σ(I)>	5.9
Completeness [%]	99.7	95.3
Redundancy	4.5	4
CC(1/2)	0.992	0.408

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	17% PEG 3350, 0.1 M HEPES pH 6.5, 0.15 M (NH4)2SO4