6WVV
Plasmodium vivax M17 leucyl aminopeptidase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-03-28 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9537 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 117.035, 201.549, 166.224 |
Unit cell angles | 90.00, 106.01, 90.00 |
Refinement procedure
Resolution | 44.320 - 2.330 |
R-factor | 0.2073 |
Rwork | 0.205 |
R-free | 0.24350 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3kqz |
RMSD bond length | 0.003 |
RMSD bond angle | 0.497 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.950 | 2.330 |
High resolution limit [Å] | 2.290 | 2.290 |
Rmerge | 0.154 | |
Rmeas | 0.174 | |
Rpim | 0.081 | |
Number of reflections | 327356 | 15478 |
<I/σ(I)> | 5.9 | |
Completeness [%] | 99.7 | 95.3 |
Redundancy | 4.5 | 4 |
CC(1/2) | 0.992 | 0.408 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 17% PEG 3350, 0.1 M HEPES pH 6.5, 0.15 M (NH4)2SO4 |