6WP1

The Crystal Structure of Apo Domain-Swapped Trimer Q108K:K40L:T51K Variant of HCRBPII

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2019-06-29
Detector	RAYONIX MX300HE
Wavelength(s)	0.97872
Spacegroup name	P 21 21 21
Unit cell lengths	69.831, 78.466, 178.887
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.480 - 2.990
R-factor	0.252
Rwork	0.247
R-free	0.30200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2rct
RMSD bond length	0.002
RMSD bond angle	0.578
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.480	3.030
High resolution limit [Å]	2.986	2.986
Rmeas	0.292	1.180
Number of reflections	20546	1916
<I/σ(I)>	13.1	2.3
Completeness [%]	100.0
Redundancy	13.6	11.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	PEG4000, sodium acetate, ammonium acetate