6WNO
Plasmodium vivax reticulocyte binding protein 2b (PvRBP2b) bound to human monoclonal antibody 243244
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-10-17 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9537 |
Spacegroup name | P 1 |
Unit cell lengths | 61.010, 84.440, 90.910 |
Unit cell angles | 90.73, 110.12, 103.09 |
Refinement procedure
Resolution | 42.996 - 3.350 |
R-factor | 0.2466 |
Rwork | 0.245 |
R-free | 0.28350 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5w53 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.15_3459) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 42.996 | 42.996 | 3.440 |
High resolution limit [Å] | 3.350 | 14.980 | 3.350 |
Rmerge | 0.182 | 0.044 | 0.838 |
Rmeas | 0.216 | 0.052 | 0.988 |
Total number of observations | 81267 | ||
Number of reflections | 23223 | 250 | 1674 |
<I/σ(I)> | 6.9 | 24.66 | 1.59 |
Completeness [%] | 97.7 | 94.3 | 97.4 |
Redundancy | 3.499 | 3.4 | 3.47 |
CC(1/2) | 0.989 | 0.997 | 0.685 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 295 | 0.2 M trimethylamine N-oxide, 0.1 M Tris chloride pH 8.5, 20% (w/v) PEG monomethyl ether 2,000 |