6WN4

Structural basis for the binding of monoclonal antibody 5D2 to the tryptophan-rich lipid-binding loop in lipoprotein lipase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-25
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9201
Spacegroup name	P 1 21 1
Unit cell lengths	51.624, 67.745, 130.073
Unit cell angles	90.00, 94.37, 90.00

Refinement procedure

Resolution	29.264 - 2.800
Rwork	0.208
R-free	0.24270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5d8j
RMSD bond length	0.003
RMSD bond angle	1.263
Data reduction software	HKL-2000 (v720)
Data scaling software	HKL-2000 (v720)
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.264	2.840
High resolution limit [Å]	2.740	2.740
Rmerge	0.120	0.692
Rmeas	0.133	0.769
Rpim	0.057	0.330
Number of reflections	23348	2178
<I/σ(I)>	10.4	1.9
Completeness [%]	99.3	93.72
Redundancy	5.1	5.08
CC(1/2)	0.995	0.729

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	16-22% PEG 3350, 250mM Sodium Thiocyanate