6WMZ

Crystal structure of human SFPQ/NONO complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-07-26
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.954
Spacegroup name	P 21 21 21
Unit cell lengths	66.630, 112.940, 204.620
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	63.436 - 2.850
Rwork	0.236
R-free	0.28260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4wii
RMSD bond length	0.003
RMSD bond angle	1.241
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	68.210	2.980
High resolution limit [Å]	2.850	2.850
Number of reflections	36690	4446
<I/σ(I)>	10.2
Completeness [%]	99.5
Redundancy	4.7
CC(1/2)	0.996	0.627

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Tris-HCl (pH 8.5), 0.2 M ammonium sulfate, 19% PEG 3350