6WMH
Next generation monomeric IgG4 Fc
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-05-12 |
Detector | DECTRIS PILATUS 300K |
Wavelength(s) | 1.19499 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 102.974, 110.925, 87.175 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.000 - 2.300 |
R-factor | 0.2028 |
Rwork | 0.201 |
R-free | 0.24310 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5hvw |
RMSD bond length | 0.013 |
RMSD bond angle | 1.876 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.169 | 1.282 |
Rpim | 0.069 | 0.528 |
Number of reflections | 22417 | 2147 |
<I/σ(I)> | 7.4 | |
Completeness [%] | 99.2 | 98.2 |
Redundancy | 6.8 | 6.7 |
CC(1/2) | 0.987 | 0.599 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 295 | DL-Glutamic acid monohydrate, DL-Alanine, Glycine, DL-Lysine monohydrochloride, DL-Serine, Sodium HEPES, MOPS (acid), Ethylene glycol, PEG 8000 |