6WME
Human Sun2-KASH3 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-31 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 78.709, 78.709, 173.278 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 63.430 - 1.530 |
| R-factor | 0.2108 |
| Rwork | 0.209 |
| R-free | 0.24490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4dxt |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.056 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17_3644) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.430 | 1.570 |
| High resolution limit [Å] | 1.530 | 1.530 |
| Rpim | 0.014 | |
| Number of reflections | 47679 | 2783 |
| <I/σ(I)> | 68.3 | |
| Completeness [%] | 99.9 | 82 |
| Redundancy | 20 | |
| CC(1/2) | 0.999 | 0.753 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 16-18% PEG 3350, 0.2M magnesium chloride, 0.1M ammonium citrate, 0.1M BisTris/HCl, 0.01M nickel chloride |
