6WM8

Proliferation-Associated protein 2G4 (PA2G4)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2017-08-10
Detector	ADSC QUANTUM 315
Wavelength(s)	0.956
Spacegroup name	P 41 21 2
Unit cell lengths	138.368, 138.368, 220.209
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.920 - 2.600
R-factor	0.19643
Rwork	0.194
R-free	0.23785
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2q8k
RMSD bond length	0.014
RMSD bond angle	2.035
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.920	2.660
High resolution limit [Å]	2.600	2.600
Rmerge	0.133	1.414
Rmeas	0.133	1.467
Rpim	0.036	0.389
Number of reflections	66388	4395
<I/σ(I)>	14.6	2.1
Completeness [%]	100.0	100
Redundancy	13.7	14.1
CC(1/2)	0.998	0.730

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293	2.4M Ammonium Sulphate 100 sodium citrate pH 5.0