6WK0

Crystal structure of human ribokinase in complex with AMPPCP and ribose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2009-02-04
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	52.020, 89.350, 144.700
Unit cell angles	90.00, 98.61, 90.00

Refinement procedure

Resolution	51.430 - 2.000
R-factor	0.19888
Rwork	0.197
R-free	0.23929
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5c41
RMSD bond length	0.013
RMSD bond angle	1.771
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.430	2.030
High resolution limit [Å]	2.000	2.000
Rmerge	0.096	0.859
Rmeas	0.113	1.019
Rpim	0.059	0.541
Number of reflections	87255	4039
<I/σ(I)>	9.4	1.3
Completeness [%]	98.6	93.15
Redundancy	3.6	3.36
CC(1/2)	0.997	0.485

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	277.15	0.1 M Tris, 8% PEG 8000