6WJ4
The crystal structure of the 2009/H1N1/California PA endonuclease wild type in complex with SJ000986448
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-03-05 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.700 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 90.090, 90.090, 132.141 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 63.700 - 2.550 |
| R-factor | 0.2305 |
| Rwork | 0.228 |
| R-free | 0.27320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5vpt |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.440 | 2.660 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.060 | 0.848 |
| Rmeas | 0.065 | 0.911 |
| Rpim | 0.023 | 0.329 |
| Number of reflections | 9099 | 1072 |
| <I/σ(I)> | 16.1 | 1.9 |
| Completeness [%] | 99.2 | 98.4 |
| Redundancy | 7.2 | 7.4 |
| CC(1/2) | 0.999 | 0.879 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M HEPES PH 7.8, 1 M AMMONIUM SULFATE, 10 MM MNCL2, 10 MM MGCL2, 0.5% PVP K15 |
