6WJ4
The crystal structure of the 2009/H1N1/California PA endonuclease wild type in complex with SJ000986448
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-03-05 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.700 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 90.090, 90.090, 132.141 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 63.700 - 2.550 |
R-factor | 0.2305 |
Rwork | 0.228 |
R-free | 0.27320 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5vpt |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 74.440 | 2.660 |
High resolution limit [Å] | 2.550 | 2.550 |
Rmerge | 0.060 | 0.848 |
Rmeas | 0.065 | 0.911 |
Rpim | 0.023 | 0.329 |
Number of reflections | 9099 | 1072 |
<I/σ(I)> | 16.1 | 1.9 |
Completeness [%] | 99.2 | 98.4 |
Redundancy | 7.2 | 7.4 |
CC(1/2) | 0.999 | 0.879 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M HEPES PH 7.8, 1 M AMMONIUM SULFATE, 10 MM MNCL2, 10 MM MGCL2, 0.5% PVP K15 |