6WIY
Crystal structure of Fab 54-1G05
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-10-10 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.0332 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 60.909, 79.092, 107.645 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.258 - 1.650 |
R-factor | 0.1593 |
Rwork | 0.158 |
R-free | 0.18790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4fqh |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.710 |
High resolution limit [Å] | 1.650 | 3.550 | 1.650 |
Rmerge | 0.077 | 0.038 | 0.862 |
Rmeas | 0.080 | 0.040 | 0.899 |
Rpim | 0.022 | 0.011 | 0.252 |
Total number of observations | 802134 | ||
Number of reflections | 62699 | 6655 | 6106 |
<I/σ(I)> | 4.9 | ||
Completeness [%] | 99.3 | 100 | 98.3 |
Redundancy | 12.8 | 12.6 | 12.3 |
CC(1/2) | 0.999 | 0.839 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% PEG 8000, 0.2 M NaCl, and 0.1 M phosphate-citrate pH 4.2 |