6WIX
Crystal Structure of HIV-1 MI369 RnS-DS.SOSIP Prefusion Env Trimer in Complex with Human Antibodies 3H109L and 35O22 at 3.5 Angstrom
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-12-07 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 1 |
Spacegroup name | P 63 |
Unit cell lengths | 131.860, 131.860, 316.390 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.390 - 2.670 |
R-factor | 0.2271 |
Rwork | 0.225 |
R-free | 0.26970 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4tvp |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.600 |
High resolution limit [Å] | 2.560 | 6.940 | 2.560 |
Rmerge | 0.097 | 0.027 | 2.100 |
Rmeas | 0.111 | 0.031 | 2.526 |
Rpim | 0.053 | 0.015 | 1.380 |
Total number of observations | 236565 | ||
Number of reflections | 62697 | 5026 | 729 |
<I/σ(I)> | 5.9 | ||
Completeness [%] | 62.7 | 99.3 | 14.6 |
Redundancy | 3.8 | 4 | 2 |
CC(1/2) | 0.997 | 0.141 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 298 | 0.18M LiSO4, 0.09M Imidazole pH 6.5, 4.5% MPD, 7.425% PEG3350 |