6WIE
Post-catalytic nicked complex of human Polymerase Mu on a complementary DNA double-strand break substrate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 93 |
Detector technology | PIXEL |
Collection date | 2019-12-05 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 60.073, 62.247, 118.318 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.900 - 1.500 |
R-factor | 0.1704 |
Rwork | 0.170 |
R-free | 0.18680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4m04 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.030 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.530 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmeas | 0.116 | 0.636 |
Rpim | 0.047 | 0.266 |
Number of reflections | 71986 | 3544 |
<I/σ(I)> | 18.5 | 1.77 |
Completeness [%] | 99.8 | 99.8 |
Redundancy | 6.3 | 5.4 |
CC(1/2) | 0.995 | 0.807 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 277 | 40-45.5 mM MES, pH 5.6, 0.16-0.182 M potassium chloride, 8.2-9.1 mM magnesium sulfate, 8.2-9.1% w/v PEG400 |