6WIB

Next generation monomeric IgG4 Fc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-08-25
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	1.19499
Spacegroup name	C 2 2 21
Unit cell lengths	101.306, 101.731, 69.044
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.920 - 2.550
R-factor	0.2193
Rwork	0.217
R-free	0.26560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5hvw
RMSD bond length	0.009
RMSD bond angle	1.739
Data reduction software	XDS
Data scaling software	pointless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.920	2.660
High resolution limit [Å]	2.550	2.550
Rmerge	0.154	0.958
Rpim	0.061	0.377
Number of reflections	11992	1429
<I/σ(I)>	11.8	2.3
Completeness [%]	99.9
Redundancy	7.3
CC(1/2)	0.995	0.800

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	298	0.01 M zinc sulfate heptahydrate; 0.1 M morpholineethanesulfonic acid (MES) monohydrate, pH 6.5, and 25% (w/v) PEG 550 MME at a protein concentration of 5.5 mg/mL