6WHK

Minimally mutated anti-influenza broadly neutralizing antibody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	69.570, 76.330, 126.170
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	63.080 - 2.600
R-factor	0.2204
Rwork	0.218
R-free	0.26750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hkx
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	63.080	63.080	2.700
High resolution limit [Å]	2.600	50.000	2.600
Rmerge	0.163	0.011	1.577
Rmeas	0.181	0.015	1.743
Total number of observations	119011
Number of reflections	21307	3	2235
<I/σ(I)>	10.94	8.5	1.35
Completeness [%]	100.0	75	100
Redundancy	5.586	2.333	5.579
CC(1/2)	0.994	1.000	0.413

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG 3350