6WHE
Structure of phosphomimetic Rab8a GTPase (T72E) in the GTP-bound state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-2 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-09-30 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979279 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.092, 118.377, 39.603 |
| Unit cell angles | 90.00, 101.96, 90.00 |
Refinement procedure
| Resolution | 29.290 - 1.730 |
| R-factor | 0.1888 |
| Rwork | 0.187 |
| R-free | 0.23040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lhw |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.859 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.290 | 1.760 |
| High resolution limit [Å] | 1.720 | 1.720 |
| Rmerge | 0.135 | 0.441 |
| Rmeas | 0.154 | 0.510 |
| Rpim | 0.072 | 0.250 |
| Number of reflections | 144140 | 1551 |
| <I/σ(I)> | 6.9 | |
| Completeness [%] | 96.0 | 85.2 |
| Redundancy | 4.4 | 3.9 |
| CC(1/2) | 0.986 | 0.806 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 289 | 20% PEG3350, 0.1M Hepes pH 7 |
