6WHE
Structure of phosphomimetic Rab8a GTPase (T72E) in the GTP-bound state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-2 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-09-30 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.979279 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 36.092, 118.377, 39.603 |
Unit cell angles | 90.00, 101.96, 90.00 |
Refinement procedure
Resolution | 29.290 - 1.730 |
R-factor | 0.1888 |
Rwork | 0.187 |
R-free | 0.23040 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4lhw |
RMSD bond length | 0.006 |
RMSD bond angle | 0.859 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.290 | 1.760 |
High resolution limit [Å] | 1.720 | 1.720 |
Rmerge | 0.135 | 0.441 |
Rmeas | 0.154 | 0.510 |
Rpim | 0.072 | 0.250 |
Number of reflections | 144140 | 1551 |
<I/σ(I)> | 6.9 | |
Completeness [%] | 96.0 | 85.2 |
Redundancy | 4.4 | 3.9 |
CC(1/2) | 0.986 | 0.806 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 289 | 20% PEG3350, 0.1M Hepes pH 7 |