6WH1
Structure of the complex of human DNA ligase III-alpha and XRCC1 BRCT domains
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 12.3.1 |
| Synchrotron site | ALS |
| Beamline | 12.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-04-09 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 1.12712 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 88.073, 88.073, 43.039 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.480 - 2.400 |
| R-factor | 0.226 |
| Rwork | 0.221 |
| R-free | 0.27200 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.693 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | PHENIX (1.17.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.483 | 2.460 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 6860 | 376 |
| <I/σ(I)> | 29.9 | |
| Completeness [%] | 98.4 | 97.8 |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 295 | 8-10% isopropanol and 0.1M Bis-Tris pH 5.5 |
