6WB9
Structure of the S. cerevisiae ER membrane complex
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3 |
| Number of Particle | 590118 |
| Software | Coot,RELION,UCSF Chimera,SerialEM,CTFFIND,PHENIX |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
