6WAX
C-terminal SH2 domain of p120RasGAP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-14 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97920 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.499, 65.588, 71.614 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.368 - 1.500 |
| R-factor | 0.1799 |
| Rwork | 0.179 |
| R-free | 0.19680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1d4t |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.872 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 3.230 | 1.500 |
| Rmerge | 0.095 | 0.073 | 1.026 |
| Rmeas | 0.099 | 0.076 | 1.106 |
| Rpim | 0.028 | 0.021 | 0.407 |
| Total number of observations | 471661 | ||
| Number of reflections | 40194 | 4270 | 3941 |
| <I/σ(I)> | 7.4 | ||
| Completeness [%] | 100.0 | 100 | 99.9 |
| Redundancy | 11.7 | 12.5 | 7 |
| CC(1/2) | 0.997 | 0.689 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 2.3 M Ammonium Sulfate, 0.1 M Bis Tris pH 6.5 |
