6WAV
Crystal structure of PHF1 in complex with H3K36me3 substitution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-06-08 |
Detector | RIGAKU SATURN A200 |
Wavelength(s) | 1.54 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 153.003, 65.616, 29.026 |
Unit cell angles | 90.00, 97.12, 90.00 |
Refinement procedure
Resolution | 32.802 - 1.700 |
Rwork | 0.197 |
R-free | 0.24070 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4hcz |
RMSD bond length | 0.011 |
RMSD bond angle | 1.664 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.070 | 40.070 | 1.730 |
High resolution limit [Å] | 1.700 | 8.990 | 1.700 |
Rmerge | 0.048 | 0.031 | 0.416 |
Rmeas | 0.056 | 0.038 | 0.487 |
Rpim | 0.029 | 0.021 | 0.251 |
Total number of observations | 107846 | 706 | 5410 |
Number of reflections | 29381 | 219 | 1501 |
<I/σ(I)> | 16.4 | 34.3 | 3.1 |
Completeness [%] | 93.8 | 94.5 | 90.9 |
Redundancy | 3.7 | 3.2 | 3.6 |
CC(1/2) | 0.998 | 0.998 | 0.810 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 291 | 1.2 M ammonium sulfate and 0.1 M sodium acetate 4.6 |