6W9V
Structure of human MAIT A-F7 TCR in complex with patient MR1-R9H without ligand
Experimental procedure
Experimental method | LAUE |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-07-06 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9536 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 216.990, 70.405, 142.901 |
Unit cell angles | 90.00, 104.15, 90.00 |
Refinement procedure
Resolution | 46.190 - 1.950 |
R-factor | 0.1625 |
Rwork | 0.160 |
R-free | 0.20010 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6puc |
RMSD bond length | 0.013 |
RMSD bond angle | 1.170 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.190 | 2.020 |
High resolution limit [Å] | 1.950 | 1.950 |
Number of reflections | 152021 | 15086 |
<I/σ(I)> | 10.3 | |
Completeness [%] | 99.6 | |
Redundancy | 3.5 | |
CC(1/2) | 0.997 | 0.592 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.3 | 298 | PEG3350, BTP, Cacl2 |