6W9V

Structure of human MAIT A-F7 TCR in complex with patient MR1-R9H without ligand

Experimental procedure

Experimental method	LAUE
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9536
Spacegroup name	C 1 2 1
Unit cell lengths	216.990, 70.405, 142.901
Unit cell angles	90.00, 104.15, 90.00

Refinement procedure

Resolution	46.190 - 1.950
R-factor	0.1625
Rwork	0.160
R-free	0.20010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6puc
RMSD bond length	0.013
RMSD bond angle	1.170
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.190	2.020
High resolution limit [Å]	1.950	1.950
Number of reflections	152021	15086
<I/σ(I)>	10.3
Completeness [%]	99.6
Redundancy	3.5
CC(1/2)	0.997	0.592

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.3	298	PEG3350, BTP, Cacl2