6W8J

Structure of DNMT3A (R882H) in complex with CAG DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-07
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.5
Spacegroup name	H 3
Unit cell lengths	205.943, 205.943, 89.371
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.346 - 2.445
R-factor	0.1847
Rwork	0.183
R-free	0.22260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5XY2
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.350	2.540
High resolution limit [Å]	2.445	2.450
Number of reflections	52247	51900
<I/σ(I)>	7.8
Completeness [%]	100.0
Redundancy	4.8
CC(1/2)	0.992	0.404

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.1 M Sodium Citrate pH 4.4, 0.2 % PEG8000